The resultant plasmid was named pYlalg3PUT-ALG6. Equally, pYlalg3PLT-ALG6 was manufactured by exchanging the URA3 cassette in pYlalg3PUT-ALG6 with the LEU2 assortment marker from pKS-LPR-LEU2 be means of I-SceI digestion
T. brucei GII and mutanase tested as engineering approach. (A) The dual N-glycosylation program...