Y J, Thorup T, et al. Molecular mapping of capsaicinoid biosynthesis genes and quantitative trait loci evaluation for capsaicinoid content in Capsicum. Theoretical and Applied Genetics 108: 79 86. 19. Kim M, Kim S, Kim S, Kim B-D Isolation of cDNA clones differentially accumulated inside the placenta of pungent pepper by suppression subtractive hybridization. Molecules and Cells 11: 213219. 20. Stewart C, Kang B-C, Liu K, Mazourek M, Moore SL, et al. The Pun1 gene for pungency in pepper encodes a putative acyltransferase. The Plant Journal 42: 675688. 21. Stewart C, Mazourek M, Stellari GM, O’Connell MA, Jahn M Genetic handle of pungency in C. chinense by way of the Pun1 locus. Journal of Experimental Botany 58: 979991. 22. Stellari GM, Mazourek M, Jahn MM Contrasting modes for loss of pungency between cultivated and wild species of Capsicum. Heredity 104: 460 471. 23. Hill TA, Ashrafi H, Reyes-Chin-Wo S, Yao J, Stoffel K, et al. Characterization of Capsicum annuum Genetic Diversity and Population Structure Based on Parallel Polymorphism Discovery using a 30K Unigene Pepper GeneChip. PLoS 1 8: e56200. 24. Han K, Jeong H-J, Sung J, Keum Y, Cho M-C, et al. Biosynthesis of capsinoid is controlled by the Pun1 locus in pepper. Molecular Breeding 31: 537548. 25. Yumnam J, Tyagi W, Pandey A, Meetei NT, Rai M Evaluation of Genetic Diversity of Chilli Landraces from North Eastern India Depending on 26. 27. Morphology, SSR Markers and the Pun1 Locus. Plant Molecular Biology Reporter 30: 14701479. Bennett DJ, Kirby GW Constitution and biosynthesis of capsaicin. Journal on the Chemical Society C: Organic: 442446. Collins MD, Wasmund LM, Bosland PW Enhanced strategy for quantifying capsaicinoids in Capsicum applying high-performance liquid chromatography. HortScience 30: 137139. Zewdie Y, Bosland PW Capsaicinoid profiles are usually not great chemotaxonomic indicators for Capsicum species. Biochemical Systematics and Ecology 29: 161169. Iwai K, Suzuki T, Fujiwake H Formation and accumulation of pungent principle of hot pepper fruits, capsaicin and its analogues, in Capsicum annuun var. annuun cv. Karayatsubusa at diverse development stages immediately after flowering. Agricultural and Biological Chemistry 43: 24932498. Rozen S, Anlotinib Skaletsky HJ Primer3. Available: http://biotoolsumassmededu/ bioapps/primer3_wwwcgi. Wheelan SJ, Church DM, Ostell JM Spidey: a tool for mRNA-togenomic alignments. Genome Investigation 11: 19521957. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, et al. MEGA5: Molecular Evolutionary Genetics Analysis working with Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution 28: 27312739. Librado P, Rozas J DnaSP v5: a computer software for JI 101 extensive evaluation of DNA polymorphism information. Bioinformatics 25: 14511452. Higo K, Ugawa Y, Iwamoto M, Korenaga T Plant cis-acting regulatory DNA elements database: 1999. Nucleic Acids Study 27: 297300. Gabriel SB, Schaffner SF, Nguyen H, Moore JM, Roy J, et al. The 12926553 Structure of Haplotype Blocks within the Human Genome. Science 296: 22252229. Fallin D, Schork NJ Accuracy of Haplotype Frequency Estimation for Biallelic Loci, by way of the Expectation-Maximization Algorithm for Unphased Diploid Genotype Data. The American Journal of Human Genetics 67: 947 959. Abburi L Linkage disequilibrium and population structure analysis among Capsicum annuum L. cultivars for use in association mapping. WV, USA: West Virginia State University. Holm S A simple sequentially rejective many test process. Scandinavian Journ.Y J, Thorup T, et al. Molecular mapping of capsaicinoid biosynthesis genes and quantitative trait loci evaluation for capsaicinoid content material in Capsicum. Theoretical and Applied Genetics 108: 79 86. 19. Kim M, Kim S, Kim S, Kim B-D Isolation of cDNA clones differentially accumulated inside the placenta of pungent pepper by suppression subtractive hybridization. Molecules and Cells 11: 213219. 20. Stewart C, Kang B-C, Liu K, Mazourek M, Moore SL, et al. The Pun1 gene for pungency in pepper encodes a putative acyltransferase. The Plant Journal 42: 675688. 21. Stewart C, Mazourek M, Stellari GM, O’Connell MA, Jahn M Genetic handle of pungency in C. chinense via the Pun1 locus. Journal of Experimental Botany 58: 979991. 22. Stellari GM, Mazourek M, Jahn MM Contrasting modes for loss of pungency among cultivated and wild species of Capsicum. Heredity 104: 460 471. 23. Hill TA, Ashrafi H, Reyes-Chin-Wo S, Yao J, Stoffel K, et al. Characterization of Capsicum annuum Genetic Diversity and Population Structure Determined by Parallel Polymorphism Discovery using a 30K Unigene Pepper GeneChip. PLoS One particular 8: e56200. 24. Han K, Jeong H-J, Sung J, Keum Y, Cho M-C, et al. Biosynthesis of capsinoid is controlled by the Pun1 locus in pepper. Molecular Breeding 31: 537548. 25. Yumnam J, Tyagi W, Pandey A, Meetei NT, Rai M Evaluation of Genetic Diversity of Chilli Landraces from North Eastern India Based on 26. 27. Morphology, SSR Markers along with the Pun1 Locus. Plant Molecular Biology Reporter 30: 14701479. Bennett DJ, Kirby GW Constitution and biosynthesis of capsaicin. Journal of your Chemical Society C: Organic: 442446. Collins MD, Wasmund LM, Bosland PW Improved process for quantifying capsaicinoids in Capsicum applying high-performance liquid chromatography. HortScience 30: 137139. Zewdie Y, Bosland PW Capsaicinoid profiles are not superior chemotaxonomic indicators for Capsicum species. Biochemical Systematics and Ecology 29: 161169. Iwai K, Suzuki T, Fujiwake H Formation and accumulation of pungent principle of hot pepper fruits, capsaicin and its analogues, in Capsicum annuun var. annuun cv. Karayatsubusa at diverse development stages immediately after flowering. Agricultural and Biological Chemistry 43: 24932498. Rozen S, Skaletsky HJ Primer3. Readily available: http://biotoolsumassmededu/ bioapps/primer3_wwwcgi. Wheelan SJ, Church DM, Ostell JM Spidey: a tool for mRNA-togenomic alignments. Genome Investigation 11: 19521957. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, et al. MEGA5: Molecular Evolutionary Genetics Analysis making use of Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Strategies. Molecular Biology and Evolution 28: 27312739. Librado P, Rozas J DnaSP v5: a software for comprehensive analysis of DNA polymorphism information. Bioinformatics 25: 14511452. Higo K, Ugawa Y, Iwamoto M, Korenaga T Plant cis-acting regulatory DNA components database: 1999. Nucleic Acids Analysis 27: 297300. Gabriel SB, Schaffner SF, Nguyen H, Moore JM, Roy J, et al. The 12926553 Structure of Haplotype Blocks within the Human Genome. Science 296: 22252229. Fallin D, Schork NJ Accuracy of Haplotype Frequency Estimation for Biallelic Loci, via the Expectation-Maximization Algorithm for Unphased Diploid Genotype Data. The American Journal of Human Genetics 67: 947 959. Abburi L Linkage disequilibrium and population structure analysis amongst Capsicum annuum L. cultivars for use in association mapping. WV, USA: West Virginia State University. Holm S A basic sequentially rejective a number of test process. Scandinavian Journ.