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Testosterone ELISA kit
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Easy-to-use, highly sensitive ELISA kit for measuring testosterone with results in 3h Sensitive measurement of testosterone, detecting as little as 5.67 pg/mlSimple and ready-to-use liquid color-coded reagents reduces errorSteroid displacement reagent provided to assay free or total testosteroneHigh throughput format with results in 3 hoursExcellent consistency lot-after-lot ideal for longitudinal studiesFully quantitative results that surpass semi-quantitative Western blot analysis The Testosterone ELISA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. Ready-to-use liquid color-coded reagents are provided to reduce error. The reproducible results lot-after-lot provide excellent consistency, ideal for longitudinal studies.Testosterone is one of the most important androgens secreted into the bloodstream and is synthesized from pregnenolone which is itself formed from cholesterol. Testosterone is the main androgen secreted by the Leydig cells of the testes and effects both primary and secondary sexual development such as muscle mass and sex drive. In adult humans, testosterone circulates in plasma predominately bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin. Testosterone ELISA kit standard curve Testosterone ELISA kit standard curve
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| Application Colorimetric detection, ELISA | Application Notes For the quantitative determination of testosterone in culture supernatants, plasma, serum, and saliva from any species.1448671-31-5 Technical Information Not suitable for human serum and plasma.1965291-08-0 site For those samples use product ADI-900-176.PMID:20301593 Cited sample types include tissue and feces. | Assay Time 3 hours | Contents GxM IgG Microtiter plate, Conjugate, Antibody, Assay buffer 3, Wash buffer concentrate, Standard, Steroid displacement reagent, pNpp Substrate, Stop solution | Crossreactivity Testosterone (100%), 19-hydroxytestosterone (14.6%), Androstendione (7.20%), Dehydroepiandrosterone (0.72%), Estradiol (0.40%) and | Sensitivity 5.67 pg/ml (range 7.81 – 2,000 pg/ml) | Species Reactivity Species independent | Technical Info / Product Notes Cited samples:Hormone ELISAs: Cited SamplesWildlife Endocrinology: Cited Sample TypesApplication Note:Validation of Two Steroid Hormone ELISA Kits with Ovine Blood Samples. | Wavelength 405 nm
