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PGF2α receptor (FP) polyclonal antibody
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Arachidonic acid is converted by cyclooxygenase (COX) to prostaglandin H2 (PGH2), which is further converted to the derivative prostanoids PGD2, PGE2, PGF2α, PGI2 (prostacyclin) and thromboxane A2 (TXA2). Five classes of prostanoid receptors are recognized and named DP, EP, FP, IP and TP receptors corresponding to the agonist properties of the associated prostanoids.{{149647-78-9} MedChemExpress|{149647-78-9} Purity & Documentation|{149647-78-9} In stock|{149647-78-9} custom synthesis} FP receptors are found in smooth muscle where they mediate contraction, and are believed to be coupled via Gq/11 subunits to stimulate phosphatidyl inositol (PI) hydrolysis.{{25535-16-4} site|{25535-16-4} Biological Activity|{25535-16-4} Description|{25535-16-4} manufacturer} The FP agonist Fluprostenol (a PGF2α analogue) is used in animal husbandry to synchronize oestrus and induce partruition. FP receptors are known to mediate intraocular pressure, and FP agonists such as latanoprost and travoprost are drugs commonly use to treat glaucoma.PMID:30571054 Western blot analysis of MW marker (1) and 25µg rat kidney extract (2), probed with PGF2α Receptor (FP) pAb. Membrane ELISA: Lewis rat striatum membranes (5µg/well) were treated with 1 μM concentrations of agonist (cyclosin) and probed with PGF2α Receptor (FP) pAb (1:250 to 1:2000 of a 1µg/μl stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=4). Western blot analysis of MW marker (1) and 25µg rat kidney extract (2), probed with PGF2α Receptor (FP) pAb. Membrane ELISA: Lewis rat striatum membranes (5µg/well) were treated with 1 μM concentrations of agonist (cyclosin) and probed with PGF2α Receptor (FP) pAb (1:250 to 1:2000 of a 1µg/μl stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=4).
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| Alternative Name PTGFR, Prostaglandin F2α receptor | Application ELISA, IHC, WB | Application Notes Suitable for membrane-based ELISA.Predicted MW of ~41kDa, observed by Western blot ~45kDa. Higher MW species (~60kDa) and intermediates also observed by Western blot which reflect post-translational modification of the receptor. | Crossreactivity Predicted species reactivity based on epitope sequence homology: wild boar, dog. | Formulation Liquid. In PBS containing 50% glycerol and 0.01% sodium azide. | Host Rabbit | Immunogen Synthetic peptide corresponding to the sequence near the N-terminus of rat PGF2 Receptor. | Species Reactivity Mouse, Rat | UniProt ID P43118 | Unit of Measure (UM) µg
