Hosphokinase Heme/hemoglobin uptake outer membrane receptor PhuR Transcriptional regulator PchR Pyochelin biosynthetic protein PchG UDP-2-acetamido-2-deoxy-D-glucuronic acid 3dehydrogenase, WbpB Major intrinsic various antibiotic resistance efflux outer membrane protein OprM Bifunctional UDP-glucuronic acid decarboxylase/UDP-4amino-4-deoxy-L-arabinose formyltransferase Putative ATP-dependent protease Putative transcriptional regulator Salicylate biosynthesis isochorismate synthase tRNA pseudouridine synthase A Multifunctional aminopeptidase A UDP-glucose/GDP-mannose dehydrogenase ATP-dependent protease ATP-binding subunit HslU pchF gene product RND multidrug efflux transporter MexB Putative outer membrane protein Phosphoglucosamine mutase Glycine dehydrogenase Rhamnosyltransferase chain B Epoxide hydrolase Hypothetical protein O1Q_03368 Putative ABC transporter ATP-binding protein Hypothetical protein O1Q_04078 Glucose dehydrogenase 3-Octaprenyl-4-hydroxybenzoate carboxy-lyase Protein AmbE pctC gene product Hypothetical protein O1Q_02948 Glutamate dehydrogenase pchE gene productPeptides (95 ) 43 146 33 two 30 5 two 15 14 2 4 six 2 43 20 three 16 10 13 three 23 two 4 3 42 17 6 24 six eight 5 three 2 three 9 15 6 9 three 20 20 two 30Coverage (95 ) 53.42 54.3 34.18 five.451 22.four 20.91 45.36 24.16 13.71 15.29 13.09 17 21.21 56.55 25.52 15.54 30.37 22.78 32.16 8.006 25.28 11.11 12.39 17.54 38.59 31.42 13.87 16.eight five.067 24.76 eight.09 3.967 7.512 19.33 23.32 24.37 5.956 9.34 six.967 10.34 31.65 14.79 41.57 30.Ratio (115:114) 99.08 99.08 22.28 11.91 10.57 10.28 9.20 8.95 8.24 7.94 7.38 7.31 7.31 7.11 six.55 six.31 6.25 6.08 five.40 5.15 4.79 4.61 4.57 three.98 3.80 3.66 three.44 three.25 three.25 three.19 three.16 2.91 2.86 two.75 two.73 two.68 two.51 2.42 two.42 2.27 two.11 two.07 two.05 2.P a (115:114) five.22E3 three.19E2 2.82E4 3.28E2 1.58E2 3.68E2 3.15E2 1.20E2 three.35E3 three.79E2 two.85E2 two.24E3 3.72E2 8.95E5 1.57E4 2.34E2 9.10E3 3.03E2 1.56E3 two.80E2 1.21E4 three.86E2 four.12E2 three.Tunicamycin Epigenetic Reader Domain 39E2 2.66E2 five.69E3 4.85E2 5.80E6 3.05E3 2.64E2 three.36E2 8.00E3 three.40E3 three.59E2 3.78E3 9.43E4 9.67E3 three.72E2 4.15E2 1.66E2 two.50E3 3.07E2 three.19E2 1.85ESignificance was defined as obtaining a 115:114 abundance score of 2.0 in addition to a P value for 115:114 of GMP PAO1/plac-yhjH strain (strain 115) versus the high c-di-GMP PAO1 wspFstrain (strain 114).0.05. “115:114” refers towards the ratio with the protein’s abundance within the low c-di-of 0.15. The development curves of planktonic PAO1/pBAD-yhjH cells and dispersed biofilm cells from PAO1/pBAD-yhjH and PAO1 biofilms were measured in ABTGC medium containing 0, 0.125, and two g of colistin ml 1. The OD600 was recorded every single 15 min for five h making use of the Tecan Infinite Pro2000 microplate reader. Biofilm colistin remedy assay. The P. aeruginosa PAO1 strain was cultivated in 50-ml BD Falcon tubes containing 15 ml of ABTGC medium.Penetratin Technical Information A sterile glass cover slide (24 by 60 mm) was inserted into each Falcon tube to assistance biofilm development.PMID:24189672 Immediately after overnight incubation, slide PAO1 biofilms were washed twice with 1 ml of 0.9 NaCl and supplemented withABTGC medium containing 4 g of colistin ml 1. PAO1 biofilms incubated in ABTGC medium without the need of colistin were applied as a handle. Right after 2 h of therapy, the biofilms have been washed twice with 0.9 NaCl, stained by utilizing a Live/Dead bacterial viability kit (Invitrogen), and imaged applying fluorescence microscopy (Carl Zeiss).RESULTSConstruction of P. aeruginosa cells with unique intracellular c-di-GMP levels. We constructed P. aeruginosa strains withMay 2013 Volume 57 Numberaac.asm.orgChua et al.TABLE three Proteins whose abundance in P.
