Product Name :
Anti-Rat IgG(H+L), Goat antibody(Biotin)
Applications:
ELISA,WB,IP
Reactivity :
Rat IgG(H+L)
Conjugate:
Biotin
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-Rat IgG(H+L), Goat antibody(Biotin) is designed for detecting rat IgG specifically. Anti-Rat IgG(H+L), Goat antibody(Biotin) is based on recombinant, goat IgG Fc fused single domain antibody to rat IgG coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Rat IgG(H+L), Goat antibody(Biotin) reacts with rat IgG selectively. | Immunogen: Rat IgG | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation) | Conjugate: Biotin | Specificity: Rat IgG(H+L) | Cross-Reactivity: Does not bind to mouse IgG, rabbit IgG, goat IgG, human IgG | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300 | Storage: Store at –20 °C(Avoid freeze / thaw cycles) | Background:There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from rat. Each isotype has a different heavy chain. Rat IgG consists of four subclasses-IgG1, IgG2a, IgG2b, IgG2c. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD.{{2798856-04-7} site|{2798856-04-7} Purity & Documentation|{2798856-04-7} Formula|{2798856-04-7} custom synthesis} The common IgG is monomeric with a molecular weight of approximately 150 kD.{{2036272-55-4} web|{2036272-55-4} Biological Activity|{2036272-55-4} Formula|{2036272-55-4} custom synthesis} Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.PMID:30252260
Description2 :
Anti-Rat IgG(H+L), Goat antibody(Biotin) is designed for detecting rat IgG specifically. Anti-Rat IgG(H+L), Goat antibody(Biotin) is based on recombinant, goat IgG Fc fused single domain antibody to rat IgG coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Rat IgG(H+L), Goat antibody(Biotin) reacts with rat IgG selectively.
Immunogen:
Rat IgG
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C(Avoid freeze / thaw cycles)
Function:
ELISA： 1:5000-1:20000WB: 1:5000-1:20000IP: 1-2ug/sampleDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
