Western Blot Re-Probe Solution

Additional Information:
Product Description: This second generation of non- ?-mercabtoethanol solution will let the user to re-probe a Western Blot membrane for 4-6 times with satisfied result. Protocol: Step 1. Decide how much solution needed for the membrane, usually, 6-10 ml for one membrane. Dilute the 5 x solution with D.W into 1x working solution ( 6-10 ml). ? Put a used Western Membrane in the working solution; shake at room temperature for 10 – 15 min. if the membrane was with a stronger blot . ? Decant the used working solution. Step 2. Rinse the Membrane for 4-5 times by shaking 6-10 seconds/each with 1 X PBS-T (0.1% tween20) or other accepted Western Wash Buffer Step 3. The stripped membrane is ready to use it as a new membrane. Notes: The used Western membrane must be kept in 1x PBS or wash buffer at 4 °C, reuse within one month. For longer storage, keep a membrane at -20 °C., please strip, then wrap in plastic membrane and place in the freezer.