Vimentin Antibody

Additional Information:
|Introduction Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling |Direct ELISA, Western Blot, Immuneprecipitation, immunohistochemistry. For intra-cellular staining, use 5-10 ul/106 cells. Wash twice with PBS/Azide before adding the appropr amount of secondary fluorescent reagent. Reactivity: Human. Immunogen: Purified Human Vimentin. Purification: Ion exchange column. Form of Antibody: 1ug/ml in PBS (after reconstitution). Clone: hVimentin. Isotype: mouse IgG1. Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original v after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.