TRPC6 Antibody

Additional Information:
|Introduction TRPC6 (Transient receptor potent cation channel, subfamily C, member 6), also known as TRP6, is a transient receptor potent channel.TRPC6 is a novel member of the TRPC family. The predicted 931-amino acid protein shares 93% identity with mouse Trpc6. Northern blot analysis revealed that TRPC6 is expressed primarily in placenta, lung, spleen, ovary, and small intestine. The TRPC6 gene is mapped on 11q22.1. TRPC6 is a nonselective cation channel that is activated by d (DAG) in a membrane-delimited fashion, independently of protein kinase C. Although TRPC3, the closest structural relative of TRPC6, is activated in the same manner, human TRPC1 and mouse Trpc4 and Trpc5 were unresponsive to DAG. Immunofluorescence studies showed that most TRPC6 expression is confined to podocytes. TRPC6 was also expressed in glomerular endothel cells. Card overexpression of TRPC6 in transgenic mice resulted in heightened sensitivity to stress, a propensity for lethal card growth and heart failure, and an increase in Nfat-dependent expression of beta-myosin heavy chain (MYH7), a marker for |WB 0.1-0.5 ug/ml; IHC-P 0.5-1 ug/ml. Reactivity: Human, Rat, Mouse. Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human TRPC6, different from the related rat and mouse sequences by three amino acids. Purification: Immunogen affinity purified. Form of Antibody: Lyophilized. Reconstitution: 0.2 ml of distilled water will yield a concentration of 500 ug/ml. Isotype: Rabbit IgG. Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original v after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.