Tri-methyl Lysine Antibody

Additional Information:
|Description This rabbit polyclonal antibody is specific isolated and affinity purified from the anti-serum against chemically methylated protein antigen. The affinity purified trimethylated lysine antibodies are affinity adsorbed with N-(epsilon) mono-and di-methylated lysine. The antibodies could be utilized for detection, and quantitation of proteins with N-trimethylated lysine residues. | |Recognize proteins with trimethylation on lysine residues (N-epsilon)). Not cross-react with acetylated proteins and mono-and dimethylated proteins Applications: ELISA 1:2000, Western blot: 1:1000, Immunoprecipitation: 5 ug/mg protein sample Immunofluorescent staining: 5 ug/mL or 1:100, Immunohistochemistry: 1:100. Reactivity: Human, Rat, Mouse, Bovine, Pig. Immunogen: Methylated KLH conjugates Purification: N-(epsilon) trimethyllysine on Agarose and immunoaffinity adsorbed with N-(epsilon) methylated lysine and N-(epsilon)dimehtylated Lysine on Agarose. Form of Antibody: 500 ug/ml in PBS, 50% glycerol. Storage/Stability: Stable for 1 year at -20°C and 3 months at -20°C. For maximum recovery of the product, centrifuge the original v after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.