TDT Antibody

Background:
Terminal Deoxynucleotidyl Transferase, also known as TdT and terminal transferase, is a unique DNA polymerase without template direction catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers.1 Its gene is mapped to the region 10q23-q24.2 And TDT Cdna contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids.3 TDT may be responsible for inserting nucleotides (N regions) at the V(H)-D and D-J(H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells,transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TdT catalyses the addition of nucleotides to the 3′ terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3′-overhang, but it can also add nucleotides to blunt or recessed 3′ ends. Cobalt is a necessary cofactor. PA000243-PA1227: Anti- Terminal Deoxynucleotidyl Transferase Rabbit Polyclonal Antibody. Specificity/Sensitivity: Human. No cross reactivity with other proteins. Applications: WB 1 ug/ml Reactivity: Human. Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human TDT, identical to the related rat and mouse sequence. Purification: Immunogen affinity purified. Form of Antibody: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3. Isotype: Rabbit IgG. Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.