Superoxide Dismutase 2 Antibody

Background:
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu,Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu,Zn-SOD (EC-SOD/SOD-3) (1). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit (2). The manganese containing 80 kDa tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain (3). SOD3 is a heparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways (4). SOD4 is a copper chaperone for superoxide dismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cucofactor. PA000510-PA1776: Superoxide Dismutase 2 Polyclonal Antibody Rabbit Polyclonal Antibody. Applications: WB 0.1-0.5 ug/ml; IHC-P 0.5-1 ug/ml; ICC1 ug/ml. Reactivity: Human, Rat, Mouse. Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human SOD2, identical to the related mouse sequence and different from the related rat sequence by one amino acid. Purification: Immunogen affinity purified. Form of Antibody: Lyophilized. Reconstitution: 0.2 ml of distilled water will yield a concentration of 500 ug/ml. Isotype: Rabbit IgG. Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.