SAMDC Antibody

Background:
S-adenosylmethionine decarboxylase (AdoMet-DC), also known as S-adenosylmethionine decarboxylase proenzyme (SAMDC), is a key enzyme in polyamine biosynthesis. It is localized to chromosome region 6q21-q22. SAMDC has an unusual distribution in polysomes from cells of T lymphocyte origin. It associates predominantly with monosomes and small polysomes with none located in the preribosomal or ribonucleoprotein pool. SAMDC is a critical regulatory enzyme of the polyamine synthetic pathway, and a well-studied drug target. Since SAMDC is a key regulatory enzyme in the synthesis of spermidine and spermine, the marked increase in SAMDC activity in the neonate and the sustained high enzyme levels throughout adulthood, imply a role for these polyamines in both development and mature brain function. PA000224-PA1070: SAMDC Polyclonal Antibody Rabbit Polyclonal Antibody. Specificity/Sensitivity: Human, mouse, rat. No cross reactivity with other proteins. Applications: Western blot: At 1-2ug/ml with the appropriate system to detect SAMDC in cells and tissues. Immunohistochemistry(P): At 0.5-1 ug/ml to detect SAMDC in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F): At 0.5-1 ug/ml to detect SAMDC in formalin or acetone fixed tissues. Immunocytochemistry: At 2-3ug/ml to detect SAMDC in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required. Reactivity: Human, Mouse, Rat. Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human SAMDC, identical to the related mouse and rat sequence. Purification: Immunogen affinity purified. Form of Antibody: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3. Isotype: Rabbit IgG. Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.