Product Name :
Rat IgG2b Isotype Control Antibody
EN
Recombinant Rat IgG2b Isotype Control, Rat IgG2b Negative Control Antibody, rtIgG2b Isotype Control
Source:
Molecular Weight:
Activity :
Purity:
>95% by SDS-PAGE under reducing conditions.
Endotoxin :
LevelLess than 1 EU/mg of protein as determined by LAL method.
Formulation:
0.2 μM filtered solution of 1x PBS.
Reconstitution :
Shipping:
The recombinant rtIgG2b isotype control is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
/strong>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Form of Antibody :
Clone:
12B9.
Isotype:
rat IgG2b, kappa.
Background:
Rat IgG antibodies are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a, IgG2b, and IgG2c for the heavy chain; kappa and lamda for the light chain), of which IgG1 and kappa are the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain. Questions and Answers: Question: What is an isotype control antibody and why is it used? Answer: An ideal isotype control matches the isotype of the primary antibody used in the application but lacks binding to the target. It is normally used as a negative control to differentiate any non-specific background signal from the specific antibody signal. Different from our recombinant rat IgG2b isotype control (catalog number PA007144), other rat IgG2b kappa isotype controls against KLH, TNP, DNP, GST, HEL, His-tag, Flag, c-myc, HA, and GFP meet the requirements in some cases but do not in specific cases in the presence of the targets or cross-reactivity. Question: What is the advantage of the recombinant antibody production method compared to the hybridoma method? Answer: We use chemically defined medium for cell culturing to produce the recombinant antibody. There is significant advantage for the in vitro method. First, we do not use mouse ascites for antibody production. Rat hybridoma cell lines usually adapt poorly to in vitro conditions, and generate ascites in immunocompromised mice rather than rats. Second, although antibodies can be produced by cell culturing from over 90% of hybridomas, there is a finite and significant failure rate. The recombinant method can be of help. Third, some companies and scientists add FBS into hybridoma cell culturing medium, which results in antibody contamination. There is no such contamination in production of recombinant antibodies. Fourth, we can easily switch the isotype with different antibody constant regions, and generate mutations such as LALAPG which is used in therapeutic antibody research. It is better to use the same isotype control mutation. Question: Do you use the antibody sequences of MOPC21 (hybridoma clone number, isotype: mouse IgG1, kappa) or C1.18.4 (hybridoma clone number, isotype: mouse IgG2a, kappa) to develop your recombinant rat IgG isotype controls? Answer: Our recombinant rat IgG isotype controls including the rat IgG2b isotype control (catalog # PA007144) are not from MOPC21 nor C1.18.4 though we also produce and sell the MOPC21 and C1.18.4 rat IgG isotype controls. Please contact us and ask for MOPC21 and C1.18.4 derived recombinant rat IgG kappa isotype controls if you are interested. Other in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants: In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants In vivo Grade Recombinant Human IgG2 Isotype Control Antibody In vivo Grade Recombinant Human IgG3 Isotype Control Antibody In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody In vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody In vivo Grade Recombinant Hamster IgG2 Isotype Control Antibody In vivo Grade Recombinant IgG Fc Proteins: In vivo Grade Recombinant Human IgG1 Fc Protein (hIgG1) In vivo Grade Recombinant Human IgG2 Fc Protein (hIgG2) In vivo Grade Recombinant Human IgG4 Fc Protein (hIgG4) In vivo Grade Recombinant Mouse IgG1 Fc Protein (mIgG1) In vivo Grade Recombinant Mouse IgG2a Fc Protein (mIgG2a) In vivo Grade Recombinant Mouse IgG2b Fc Protein (mIgG2b) In vivo Grade Recombinant Rat IgG2a Fc Protein (rtIgG2a) In vivo Grade Recombinant Rat IgG2b Fc Protein (rtIgG2b) In vivo Grade Recombinant Llama IgG2b Fc Protein (lIgG2b) In vivo Grade Recombinant Rabbit IgG Fc Protein (rIgG)
Additional Information:
Rat IgG2b isotype control and mutants (natural and engineered rtIgG2b isotype controls) are useful for functional study using animal model among a number of in vitro and in vivo research applications. Rat IgG2b antibodies are more like mouse IgG2a and IgG2b, and human IgG1 antibodies, which are mostly used by the therapeutic antibody drugs. Most of rat antibodies are used to study mouse target proteins. There are more and more IgG antibody formats with Fc engineered. The format with the Fc silenced, Fc silent, or Fcs with silenced effector function is the most popular. We provide Fc effector-silent rat IgG2b isotype controls. Please contact us to ask for a quote for the engineered rat IgG2b isotype control mutants, rtIgG2b isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated rtIgG2b isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the rat IgG2b isotype control and Fc mutants are available. | In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody (rtIgG2b Isotype Control) The in vivo grade recombinant rat IgG2b isotype control antibody (rtIgG2b isotype control) was produced in mammal cells. |Immunogen: N/A. |Clone: 12B9. |Isotype: rat IgG2b, kap|an isotype-matched negative control for rat IgG2b kap|0.2 μM filtered solution of 1x PBS. |Purity: >95% by SDS-|Less than 1 EU/mg of protein as determined by LAL method. |Shipping: The recombinant rtIgG2b isotype control is shipped with ice |Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied. |Background Rat IgG antibodies are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a, IgG2b, and IgG2c for the heavy chain; kap||Question: What is an isotype control antibody and why is it used? |Answer: An ideal isotype control matches the isotype of the primary antibody used in the application but lacks binding to the target. It is normally used as a negative control to different any non-specific background signal from the specific antibody signal. Different from our recombinant rat IgG2b isotype control (catalog number |What is the advantage of the recombinant antibody production method com|We use chemically defined medium for cell culturing to produce the recombinant antibody. There is significant advantage for the in vitro method. First, we do not use mouse ascites for antibody production. Rat hybridoma cell lines usually adapt poorly to in vitro conditions, and generate ascites in immunocompromised mice rather than rats. Second, although antibodies can be produced by cell culturing from over 90% of hybridomas, there is a finite and significant failure rate. The recombinant method can be of help. Third, some com|Do you use the antibody sequences of MOPC21 (hybridoma clone number, isotype: mouse IgG1, kap|mouse IgG2a, kap|Our recombinant rat IgG isotype controls including the rat IgG2b isotype control (catalog # |In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants In vivo Grade Recombinant Human IgG2 Isotype Control Antibody In vivo Grade Recombinant Human IgG3 Isotype Control Antibody In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody In vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody In vivo Grade Recombinant Hamster IgG2 Isotype Control Antibody |In vivo Grade Recombinant IgG Fc Proteins: In vivo Grade Recombinant Human IgG1 Fc Protein (hIgG1) In vivo Grade Recombinant Human IgG2 Fc Protein (hIgG2) In vivo Grade Recombinant Human IgG4 Fc Protein (hIgG4) In vivo Grade Recombinant Mouse IgG1 Fc Protein (mIgG1) In vivo Grade Recombinant Mouse IgG2a Fc Protein (mIgG2a) In vivo Grade Recombinant Mouse IgG2b Fc Protein (mIgG2b) In vivo Grade Recombinant Rat IgG2a Fc Protein (rtIgG2a) In vivo Grade Recombinant Rat IgG2b Fc Protein (rtIgG2b) In vivo Grade Recombinant Llama IgG2b Fc Protein (lIgG2b) In vivo Grade Recombinant Rabbit IgG Fc Protein (rIgG)
Related websites: https://www.medchemexpress.com/antibodies.html
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