Unction in streptozotocin-induced diabetic mice. Am J Physiol Heart Circ Physiol. 2007; 292:67383. 42. Son NH, Park TS, Yamashita H, Yokoyama M, Huggins LA, Okajima K, Homma S, Szabolcs MJ, Huang LS, Goldberg IJ. Cardiomyocyte expression of PPARgamma results in cardiac dysfunction in mice. J Clin Invest. 2007 Oct; 117(ten):2791801. [PubMed: 17823655] 43. Li H, Ruan XZ, Powis SH, Fernando R, Mon WY, Wheeler DC, Moorhead JF, Varghese Z. EPA and DHA lower LPS-induced inflammation responses in HK-2 cells: proof to get a PPARgamma-dependent mechanism. Kidney Int. 2005 Mar.67:86774.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Cardiovasc Pharmacol. Author manuscript; offered in PMC 2014 April 01.Khan et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 1. Cardiac function in fish oil (FO) and non-purified eating plan (NPD)-fed handle (Ctrl) and MHC-ACS1 (ACS1) miceEchocardiography was utilized to assess cardiac function in 14-week old mice fed a nonpurified diet (NPD) or diets containing either high (HD) or low dose (LD) FO for 6 weeks. White bars represent handle mice and black bars represent MHC-ACS1 mice. A: Fractional shortening (FS). n=30 mice per group. 2-way ANOVA for FS: genotype P = 0.0028; eating plan P = 0.0008; genotype eating plan, P = 0.056. a, P 0.001 vs. control NPD-fed mice; b, P 0.05 vs. MHC-ACS1 NPD-fed mice (by student t-test); c, P 0.001 vs. MHC-ACS1 NPD-fed mice; d, P 0.05 vs. MHC-ACS1 LD FO-fed mice. B: Left ventricular diastolic dimension (LVDd) n=30 mice per group. Two-way ANOVA for LVDd: genotype P = 0.0025; eating plan PJ Cardiovasc Pharmacol. Author manuscript; offered in PMC 2014 April 01.Khan et al.Page= 0.059; genotype diet plan, P = 0.665. a, P 0.01 vs. handle NPD-fed mice; b, P 0.05 vs. MHC-ACS1 NPD-fed mice (by student t-test); C: Heart weight/body weight (HW/BW) ratio n=47 mice per group. Two-way ANOVA for LVDd: genotype P 0.001; diet regime P = 0.0234; genotype diet plan, P = 0.71. a, P 0.001 vs. control NPD-fed mice; b, P 0.05 vs. MHC-ACS1 NPD-fed mice;, D: Gene expression of BNP was analyzed by real-time PCR and normalized to 18S expression. n=3 mice per group. Two-way ANOVA for BNP mRNA: genotype P = 0.082; eating plan P = 0.062; genotype diet plan, P = 0.048. a, P 0.01 vs. control NPD-fed mice; b, P 0.05 vs. MHC-ACS1 NPD-fed mice; c, P 0.01 vs. MHC-ACS1 NPD-fed mice. E: Survival curve of MHC-ACS1 mice. n = 125 mice per group. a, P 0.05 vs. handle NPDfed mice; b, P 0.05 vs. MHC-ACS1 NPD-fed mice.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Cardiovasc Pharmacol. Author manuscript; readily available in PMC 2014 April 01.Khan et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 2.Abemaciclib Cardiac fibrosis and inflammationRepresentative slides in the left ventricle of eight and 14 week old handle and MHC-ACS1 mice fed either non-purified diet (NPD), purified diet (PD), LD, or HD FO stained for a: collagen deposition (blue) making use of Masson’s Trichrome (magnification 200, n = three mice per group, and quantified as fibrosis of total location.Maslinic acid 2-way ANOVA for area of fibrosis: genotype P 0.PMID:24078122 0001; diet plan P 0.0001; age P = 0.2277; genotype diet = 0.0008; genotype age = 0.4525, a, P 0.001 vs. 8-wk old NPD-fed manage mice; b, P 0.05 vs. 14-wk old MHC-ACS1 NPD-fed mice, c, P 0.05 vs. PD-fed MHC-ACS1 mice. cc, P 0.01 vs. PD-fed MHC-ACS1 mice. d, P 0.001 vs. LD-fed MHC-ACS1 mice. B: -smooth muscle actin (SMA) expression to assess for fibroblast activation in 1.
