Ts (data not shown).from 3 independents experiments and obtained 86168-78-7 chemical information similar IgE concentrations, namely 13.362.2 mg/mL, 5.060.2 mg/mL and 7.460.5 mg/mL.DiscussionThe present study established long-term culture conditions enabling the generation of large quantities of human B lymphocytes. The resulting pool of human B lymphocytes, which could be enlarged by up to 106-fold after 45 to 50 days, were polyclonal and viability was still very good (.80 ). These populations included proportions of gamma isotypes as well as kappa/lambda ratios that were comparable to those observed in human blood [26,27,30]. Overall, this in vitro culture model allowsIn vitro Expanded Switched-memory B Lymphocytes Generated IgE-secreting CellsIgE+ B lymphocytes are expected to be of very low frequency in peripheral blood B lymphocytes; however we found out that the mean concentration of IgE in the above pool of 13 supernatants was 12.562.2 mg/mL. We also tested cumulated supernatantsLarge-Scale Expansion of Human B LymphocytesFigure 5. Validation of expansion during long-term culture. Three switched-memory B lymphocyte samples were cultured as described in Fig. 1 and transferred in petri dishes to test the feasibility of increasing the culture volume up to 500 mL. (A) Expansion factors were similar to those obtained in 6-well plates (Fig. 1). (B) Culture KS 176 web volumes are shown as a function of time. (C) IgA, IgG and IgM concentrations were determined in supernatants of the three independent samples at the end of the culture. (D) Flow cytometry analyses for kappa and lambda chain expression was similar for all three independent samples. doi:10.1371/journal.pone.0051946.gthe generation of large amounts of B lymphocytes as well as their utilization for the production of IgG and/or IgA. The polyclonal progression of B lymphocytes in these 13 experiments is crucial since it opens to the possibility to have access to a large human antibody repertoire. Banchereau’s group was the first to report the culture of human B lymphocytes for as long as 10 weeks [13]. Thereafter, several groups have used CD40activation to perform long-term expansion of unsorted blood B lymphocytes for cellular immunotherapy [16,31,32,33]. Among them, Wiesner’s group has done exhaustive investigations of the resulting B lymphocyte populations. Overall, their strategy provided a B lymphocyte expansion ranging from 100- to 1000-fold after 40 days that could be maintained for up to 400 days. However, although most cultured cells were EBV-negative, their analysis of kappa/lambda ratios revealed an oligoclonal expansion of human B lymphocytes, suggesting the domination of some subsets [16]. We already showed that upon CD40-activation, ?naive B lymphocytes were prone to dominate the culture [34] and were able to inhibit memory B lymphocyte expansion [35]. In the present study, by using purified switched-memory B lymphocytes, we eliminated such negative modulation and allowed the switchedmemory to expand rapidly following high levels of CD40-CD154 interactions for up to 2 months. Besides, we observed that IgA secretion was rapidly decreasing during the three weeks of culture (data not shown). In fact, in all our cultures, IgG was dominant representing 70 to 90 of all secreted immunoglobulins suggesting that proliferation and differentiation of 18325633 IgG+ cells were steadier than that of IgA+ cells in our long-term culture conditions.However, we also observed that the proportion of IgE secretion, which may represent.Ts (data not shown).from 3 independents experiments and obtained similar IgE concentrations, namely 13.362.2 mg/mL, 5.060.2 mg/mL and 7.460.5 mg/mL.DiscussionThe present study established long-term culture conditions enabling the generation of large quantities of human B lymphocytes. The resulting pool of human B lymphocytes, which could be enlarged by up to 106-fold after 45 to 50 days, were polyclonal and viability was still very good (.80 ). These populations included proportions of gamma isotypes as well as kappa/lambda ratios that were comparable to those observed in human blood [26,27,30]. Overall, this in vitro culture model allowsIn vitro Expanded Switched-memory B Lymphocytes Generated IgE-secreting CellsIgE+ B lymphocytes are expected to be of very low frequency in peripheral blood B lymphocytes; however we found out that the mean concentration of IgE in the above pool of 13 supernatants was 12.562.2 mg/mL. We also tested cumulated supernatantsLarge-Scale Expansion of Human B LymphocytesFigure 5. Validation of expansion during long-term culture. Three switched-memory B lymphocyte samples were cultured as described in Fig. 1 and transferred in petri dishes to test the feasibility of increasing the culture volume up to 500 mL. (A) Expansion factors were similar to those obtained in 6-well plates (Fig. 1). (B) Culture volumes are shown as a function of time. (C) IgA, IgG and IgM concentrations were determined in supernatants of the three independent samples at the end of the culture. (D) Flow cytometry analyses for kappa and lambda chain expression was similar for all three independent samples. doi:10.1371/journal.pone.0051946.gthe generation of large amounts of B lymphocytes as well as their utilization for the production of IgG and/or IgA. The polyclonal progression of B lymphocytes in these 13 experiments is crucial since it opens to the possibility to have access to a large human antibody repertoire. Banchereau’s group was the first to report the culture of human B lymphocytes for as long as 10 weeks [13]. Thereafter, several groups have used CD40activation to perform long-term expansion of unsorted blood B lymphocytes for cellular immunotherapy [16,31,32,33]. Among them, Wiesner’s group has done exhaustive investigations of the resulting B lymphocyte populations. Overall, their strategy provided a B lymphocyte expansion ranging from 100- to 1000-fold after 40 days that could be maintained for up to 400 days. However, although most cultured cells were EBV-negative, their analysis of kappa/lambda ratios revealed an oligoclonal expansion of human B lymphocytes, suggesting the domination of some subsets [16]. We already showed that upon CD40-activation, ?naive B lymphocytes were prone to dominate the culture [34] and were able to inhibit memory B lymphocyte expansion [35]. In the present study, by using purified switched-memory B lymphocytes, we eliminated such negative modulation and allowed the switchedmemory to expand rapidly following high levels of CD40-CD154 interactions for up to 2 months. Besides, we observed that IgA secretion was rapidly decreasing during the three weeks of culture (data not shown). In fact, in all our cultures, IgG was dominant representing 70 to 90 of all secreted immunoglobulins suggesting that proliferation and differentiation of 18325633 IgG+ cells were steadier than that of IgA+ cells in our long-term culture conditions.However, we also observed that the proportion of IgE secretion, which may represent.
