Traocular pressure (IOP) is a major risk factor in glaucomatous optic neuropathy [1] for review and the mainstay of glaucoma treatment continues to be lowering of the IOP by pharmacological or surgical methods [2]. However, order CI-1011 retinal ganglion cell (RGC) loss and damage to the optic nerve may continue despite significant reductions in IOP [3?1]. It has been assumed that inflammatory and metabolic processes are involved in glaucomatous neuron death. Crystallins, which belong to the family of small heat shock proteins (HSPs) and comprise three major families (a, b, and c crystallins), have been found within RGCs [12,13]. Both neuroregenerative [13] and neurodegenerative [14] properties have been attributed to retinal crystallins. Specific 18334597 regulation of crystallins has been observed in the context of neurodegenerative diseases such as glaucoma [12,15]. Furthermore, human glaucoma patients exhibit increased titers of antibodies against small HSPs [14,16?7]. Crystallins may act as critical modulators in glaucoma and thus be integral to the process of glaucomatous neurodegeneration [14]. The retina and the optic nerve provide an easily accessible and relevant model with which to study central nervous system injury and postinjury repair. Experimental, genetic, and hereditarymutant animal models of glaucoma provide suitable tools with which to study the complex 1313429 process of neuronal degeneration in glaucoma [18?0]. We hypothesized that IOP elevation causes alterations in gene and protein expressions within retinal cells. Based on this hypothesis, different drugs may alter the expressions of such molecules, which can be analyzed by two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) in order to identify diseaseor treatment-associated proteins. In addition, proteomic enomic correlations may help to identify novel pharmacological targets [21,22]. Several families of drugs that aim at limiting the risk for retinal and optic nerve neuropathy are in clinical use, and all of them are designed to normalize IOP. In addition to surgical procedures, a2a [23] and b-adrenergic [24] receptor agonists, prostaglandin F2a analogues [25], and carbonic anhydrase inhibitors [26] are the most important classes of drugs used in this context [27]. Some of these drugs are suspected to act neuroprotectively by altering retinal protein metabolism and activating signaling cascades in favor of RGC MedChemExpress Microcystin-LR survival. The purpose of the present study was to identify metabolic retinal changes at the genomic and proteomic levels using 2DE, MALDI-MS, microarray analysis, quantitative real-time polymerProtein Changes in Neurodegenerationase chain reaction (qRT-PCR), Western blotting (WB), and immunohistochemistry (IHC).Methods Animals and drugsEthical statement and animals: All experiments were conducted in accordance with the Association for Research in Vision and Ophthalmology (ARVO) Statement on the Use of Animals in Ophthalmic and Vision Research. Sprague-Dawley rats were housed in a standard animal room under a 12-h light/dark cycle with food and water provided ad libitum. The ethics committee (Bezirksregierung Munster, i.e. regional government of Munster) ??specially approved this study (Permission Nr.: 8402.04.2011.A132). Animals were housed in a standard animal facility with food and water ad libitum and a 12 hrs light-dark cycle. Surgical procedures were performed unilaterally, on the left eye of rats weighing 180?.Traocular pressure (IOP) is a major risk factor in glaucomatous optic neuropathy [1] for review and the mainstay of glaucoma treatment continues to be lowering of the IOP by pharmacological or surgical methods [2]. However, retinal ganglion cell (RGC) loss and damage to the optic nerve may continue despite significant reductions in IOP [3?1]. It has been assumed that inflammatory and metabolic processes are involved in glaucomatous neuron death. Crystallins, which belong to the family of small heat shock proteins (HSPs) and comprise three major families (a, b, and c crystallins), have been found within RGCs [12,13]. Both neuroregenerative [13] and neurodegenerative [14] properties have been attributed to retinal crystallins. Specific 18334597 regulation of crystallins has been observed in the context of neurodegenerative diseases such as glaucoma [12,15]. Furthermore, human glaucoma patients exhibit increased titers of antibodies against small HSPs [14,16?7]. Crystallins may act as critical modulators in glaucoma and thus be integral to the process of glaucomatous neurodegeneration [14]. The retina and the optic nerve provide an easily accessible and relevant model with which to study central nervous system injury and postinjury repair. Experimental, genetic, and hereditarymutant animal models of glaucoma provide suitable tools with which to study the complex 1313429 process of neuronal degeneration in glaucoma [18?0]. We hypothesized that IOP elevation causes alterations in gene and protein expressions within retinal cells. Based on this hypothesis, different drugs may alter the expressions of such molecules, which can be analyzed by two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) in order to identify diseaseor treatment-associated proteins. In addition, proteomic enomic correlations may help to identify novel pharmacological targets [21,22]. Several families of drugs that aim at limiting the risk for retinal and optic nerve neuropathy are in clinical use, and all of them are designed to normalize IOP. In addition to surgical procedures, a2a [23] and b-adrenergic [24] receptor agonists, prostaglandin F2a analogues [25], and carbonic anhydrase inhibitors [26] are the most important classes of drugs used in this context [27]. Some of these drugs are suspected to act neuroprotectively by altering retinal protein metabolism and activating signaling cascades in favor of RGC survival. The purpose of the present study was to identify metabolic retinal changes at the genomic and proteomic levels using 2DE, MALDI-MS, microarray analysis, quantitative real-time polymerProtein Changes in Neurodegenerationase chain reaction (qRT-PCR), Western blotting (WB), and immunohistochemistry (IHC).Methods Animals and drugsEthical statement and animals: All experiments were conducted in accordance with the Association for Research in Vision and Ophthalmology (ARVO) Statement on the Use of Animals in Ophthalmic and Vision Research. Sprague-Dawley rats were housed in a standard animal room under a 12-h light/dark cycle with food and water provided ad libitum. The ethics committee (Bezirksregierung Munster, i.e. regional government of Munster) ??specially approved this study (Permission Nr.: 8402.04.2011.A132). Animals were housed in a standard animal facility with food and water ad libitum and a 12 hrs light-dark cycle. Surgical procedures were performed unilaterally, on the left eye of rats weighing 180?.
