Caused by OVX plus ISO. E2 and E2+G15 but not G-1 
could increase uterine weight (table 1).G-1 Treatment Decreased the Fibrosis of the Failing HeartMasson staining showed that Ebselen biological activity fibrotic areas were stained green, and the normal cardiac myocytes were stained red. The green areas were lower in Sham, OVX, OVX+E2 groups, stained sections showed increased fibrosis in OVX+ISO and OVX+ISO+E2+G15 group. OVX+ISO+G-1/E2 group has lower fibrotic areas than OVX+ISO group (figure 3).G-1 Treatment Enhanced Contraction of Myocardial CellsFirst we observed contractility of myocytes isolated from Sham, OVX, OVX+ E2, OVX+ISO, OVX+ISO+G-1, OVX+ISO+E2+G15 and OVX+ISO+E2 which incubated with vehicle medium. We found that myocardial function decreased in OVX+ISO group compared with OVX group, which reflected in decreasing of contraction amplitude, and extending of systolic and diastolic. G-1 or E2 improved myocardial function, but E2+G15 had no effect on the improvement (figure 4A, 4B and 4C). This indicated that the protection was induced by activation of G protein-coupled receptor. To further study the relationship between the protective effect of G-1 and b-AR, we incubated myocytes isolated from OVX+ISO and OVX+ISO+G-1 with CGP, ICI or CGP+ICI. And then we found that, there were significant differences between OVX+ISO and OVX+ISO+G-1 in CGP or ICI medium, but in CGP+ICI medium, we didn’t find the difference (figure 4D, 4E, 4F). These indicated that the protection of G-1 may associate with both b1 and b2 -AR.G-1 Treatment Increased the Ratio of Phosphorylated AKTIn the experiment, OVX and OVX + ISO group have lower ratio of phosphorylated AKT than the Sham or OVX+E2 group, OVX + ISO +G-1 and OVX +ISO + E2 have higher ratio of phosphorylated AKT than OVX + ISO group, there was no significant differences between OVX +ISO + E2+G15 and OVX +ISO group (figure 1).G-1 Treatment Decreased BNP Levels in Heart Failure ModelWe assayed the concentration of brain natriuretic BIBS39 cost peptide (BNP) (in mg/L) in plasma before the animals were sacrificed. Ovariectomy induced a slight increase in the release of BNP compared with the Sham or OVX+E2 group. After ISO treatment, BNP release increased in all groups, but the OVX+ISO+G1/E2 group had a lower BNP release than OVX+ISO group (figure 2).b1- and b2-AR Protein Expressions in Ventricular MyocytesOur research showed that the protective effect of G-1 in heart failure can be abolished by CGP+ICI, which indicated that the protective effect of G-1 may be relevant to b-AR, so we determined whether the expression of b1- or b2-AR was changed by G-1 treatment. We added two groups OVX+G-1 and OVX+E2 for ovariectomy could change the expression of b-AR [7]. We found that compared with Sham group, the expression of b1-AR increased in OVX group, G-1 or E2 treatment decreased the expression in OVX group. However OVX plus ISO treatment could also decreased the expression of b1-AR compared with OVX group, that may because sustainedG-1 Treatment Improved Cardiac Function in Heart Failure ModelHearts were equilibrated for 30 minutes with KHB (Krebs?Henseleit buffer), and then we recorded the cardiac function data of each group. We found that there were no significant differences before ISO treatment among all groups. ISO treatment decreased LVDP, 6dp/dt and RPP, and increased LVEDP significantly. Administration of G1 or E2 increased LVDP, 6dp/dt, RPP, and Table 1. General features.Sham Number of rats Body weight (g) Body length (mm) Heart weight (mg).Caused by OVX plus ISO. E2 and E2+G15 but not G-1 could increase uterine weight (table 1).G-1 Treatment Decreased the Fibrosis of the Failing HeartMasson staining showed that fibrotic areas were stained green, and the normal cardiac myocytes were stained red. The green areas were lower in Sham, OVX, OVX+E2 groups, stained sections showed increased fibrosis in OVX+ISO and OVX+ISO+E2+G15 group. OVX+ISO+G-1/E2 group has lower fibrotic areas than OVX+ISO group (figure 3).G-1 Treatment Enhanced Contraction of Myocardial CellsFirst we observed contractility of myocytes isolated from Sham, OVX, OVX+ E2, OVX+ISO, OVX+ISO+G-1, OVX+ISO+E2+G15 and OVX+ISO+E2 which incubated with vehicle medium. We found that myocardial function decreased in OVX+ISO group compared with OVX group, which reflected in decreasing of contraction amplitude, and extending of systolic and diastolic. G-1 or E2 improved myocardial function, but E2+G15 had no effect on the improvement (figure 4A, 4B and 4C). This indicated that the protection was induced by activation of G protein-coupled receptor. To further study the relationship between the protective effect of G-1 and b-AR, we incubated myocytes isolated from OVX+ISO and OVX+ISO+G-1 with CGP, ICI or CGP+ICI. And then we found that, there were significant differences between OVX+ISO and OVX+ISO+G-1 in CGP or ICI medium, but in CGP+ICI medium, we didn’t find the difference (figure 4D, 4E, 4F). These indicated that the protection of G-1 may associate with both b1 and b2 -AR.G-1 Treatment Increased the Ratio of Phosphorylated AKTIn the experiment, OVX and OVX + ISO group have lower ratio of phosphorylated AKT than the Sham or OVX+E2 group, OVX + ISO +G-1 and OVX +ISO + E2 have higher ratio of phosphorylated AKT than OVX + ISO group, there was no significant differences between OVX +ISO + E2+G15 and OVX +ISO group (figure 1).G-1 Treatment Decreased BNP Levels in Heart Failure ModelWe assayed the concentration of brain natriuretic peptide (BNP) (in mg/L) in plasma before the animals were sacrificed. Ovariectomy induced a slight increase in the release of BNP compared 
with the Sham or OVX+E2 group. After ISO treatment, BNP release increased in all groups, but the OVX+ISO+G1/E2 group had a lower BNP release than OVX+ISO group (figure 2).b1- and b2-AR Protein Expressions in Ventricular MyocytesOur research showed that the protective effect of G-1 in heart failure can be abolished by CGP+ICI, which indicated that the protective effect of G-1 may be relevant to b-AR, so we determined whether the expression of b1- or b2-AR was changed by G-1 treatment. We added two groups OVX+G-1 and OVX+E2 for ovariectomy could change the expression of b-AR [7]. We found that compared with Sham group, the expression of b1-AR increased in OVX group, G-1 or E2 treatment decreased the expression in OVX group. However OVX plus ISO treatment could also decreased the expression of b1-AR compared with OVX group, that may because sustainedG-1 Treatment Improved Cardiac Function in Heart Failure ModelHearts were equilibrated for 30 minutes with KHB (Krebs?Henseleit buffer), and then we recorded the cardiac function data of each group. We found that there were no significant differences before ISO treatment among all groups. ISO treatment decreased LVDP, 6dp/dt and RPP, and increased LVEDP significantly. Administration of G1 or E2 increased LVDP, 6dp/dt, RPP, and Table 1. General features.Sham Number of rats Body weight (g) Body length (mm) Heart weight (mg).
