E successfully developed a simple photonic suspension array for simultaneous detection of the selected pesticides. The SCCBs were proven to be effective microcarriers with a stable encoding strategy for developing high-throughput multiplex assays. The high sensitivity obtained, when compared with other techniques, was due to the enhanced binding capacity of the porous SCCB structure. The suspension array has acceptable stability, reproducibility and Madrasin accuracy. The high sensitivity and specificity of the proposed system fully satisfies the requirements of analytical fields. 10457188 The photonic suspension assay provides significant advantages over single analyte tests in terms of cost, labor, test throughput and convenience but the most significant advantage of this method is its capability for multiplex analysis. The 16574785 photonic suspension array is very promising not only for the detection of macromolecules (proteins and nucleic acids) but provides a novel pathway for analysis of small molecules such as pesticides, veterinary drugs and toxicants.the surface of SCCBs. (TIF)Figure S3 Scheme for the SCCBs based photonic suspension array. (1) Incubation for competitive binding (30 min). (2) Washing and detection. (TIF) Figure S4 The platform was used for decoding and detection of the SCCBs. (TIF) Table S1 Cross-reactivity of chemicals determined by the photonic suspension array. (DOCX)Author ContributionsConceived and designed the experiments: XW ZDM FQSG RL YPP LHY. Performed the experiments: XW ZDM FQSG RL YPP LHY. Analyzed the data: XW RL YPP LHY. Contributed reagents/materials/ analysis tools: XW RL YPP LHY. Wrote the paper: XW RL YPP LHY.
Streptococcus suis (S.suis) is an important emerging worldwide pig pathogen and zoonotic agent causing severe meningitis, pneumonia, and sepsis in pigs and also meningitis and Streptococci toxinshock-like syndrome (STSLS) in humans [1,2]. Since the first human infection report in Denmark in 1968, infections in humans were considered sporadic infections in people working with pigs or pork-derived products [3]. However, a large outbreak of S.suis serotype 2 infection that emerged in Sichuan Province, China in 2005 and resulted in 215 cases and 38 deaths among humans, has changed the perspective of the threat posed by this pathogen to human health [4]. For S.suis infection in human, intravenous penicillin G has been used to successfully treat most cases. However, penicillin-resistant strains have been isolated in 6?8 of piglets. On the other hand, the widespread use of antibiotics such as tetracycline in swine feed have been demonstrated to provide the selective pressure for rapid evolution of virulence and drug resistance in S.suis [5,6]. Up to now, S.suis vaccines for humans do not exist so far and there are also no effective vaccines available even for swine.Licochalcone A is one of the many flavonoids present in the Chinese licorice root, which is used in traditional Chinese medicine. The structure of licochalcone A was first reported in 1975, but no biological activity was described [7]. Later studies have revealed that licochalcone A exhibits antimicrobial, antioxidant and anti-inflammatory activities [8?1]. In this study, we investigated the effect and possible mechanism of licochalcone A on growth and properties of S.suis.Results Growth of Streptococcus suis in the presence of licochalcone AThe growth curves of S.suis strain 05ZYH33 is shown in Figure 1. The data of bacteria density and colony BMS-5 web forming unit (.E successfully developed a simple photonic suspension array for simultaneous detection of the selected pesticides. The SCCBs were proven to be effective microcarriers with a stable encoding strategy for developing high-throughput multiplex assays. The high sensitivity obtained, when compared with other techniques, was due to the enhanced binding capacity of the porous SCCB structure. The suspension array has acceptable stability, reproducibility and accuracy. The high sensitivity and specificity of the proposed system fully satisfies the requirements of analytical fields. 10457188 The photonic suspension assay provides significant advantages over single analyte tests in terms of cost, labor, test throughput and convenience but the most significant advantage of this method is its capability for multiplex analysis. The 16574785 photonic suspension array is very promising not only for the detection of macromolecules (proteins and nucleic acids) but provides a novel pathway for analysis of small molecules such as pesticides, veterinary drugs and toxicants.the surface of SCCBs. (TIF)Figure S3 Scheme for the SCCBs based photonic suspension array. (1) Incubation for competitive binding (30 min). (2) Washing and detection. (TIF) Figure S4 The platform was used for decoding and detection of the SCCBs. (TIF) Table S1 Cross-reactivity of chemicals determined by the photonic suspension array. (DOCX)Author ContributionsConceived and designed the experiments: XW ZDM FQSG RL YPP LHY. Performed the experiments: XW ZDM FQSG RL YPP LHY. Analyzed the data: XW RL YPP LHY. Contributed reagents/materials/ analysis tools: XW RL YPP LHY. Wrote the paper: XW RL YPP LHY.
Streptococcus suis (S.suis) is an important emerging worldwide pig pathogen and zoonotic agent causing severe meningitis, pneumonia, and sepsis in pigs and also meningitis and Streptococci toxinshock-like syndrome (STSLS) in humans [1,2]. Since the first human infection report in Denmark in 1968, infections in humans were considered sporadic infections in people working with pigs or pork-derived products [3]. However, a large outbreak of S.suis serotype 2 infection that emerged in Sichuan Province, China in 2005 and resulted in 215 cases and 38 deaths among humans, has changed the perspective of the threat posed by this pathogen to human health [4]. For S.suis infection in human, intravenous penicillin G has been used to successfully treat most cases. However, penicillin-resistant strains have been isolated in 6?8 of piglets. On the other hand, the widespread use of antibiotics such as tetracycline in swine feed have been demonstrated to provide the selective pressure for rapid evolution of virulence and drug resistance in S.suis [5,6]. Up to now, S.suis vaccines for humans do not exist so far and there are also no effective vaccines available even for swine.Licochalcone A is one of the many flavonoids present in the Chinese licorice root, which is used in traditional Chinese medicine. The structure of licochalcone A was first reported in 1975, but no biological activity was described [7]. Later studies have revealed that licochalcone A exhibits antimicrobial, antioxidant and anti-inflammatory activities [8?1]. In this study, we investigated the effect and possible mechanism of licochalcone A on growth and properties of S.suis.Results Growth of Streptococcus suis in the presence of licochalcone AThe growth curves of S.suis strain 05ZYH33 is shown in Figure 1. The data of bacteria density and colony forming unit (.
